Optimization Strategy For Fluorescence Multiplex Immunohistochemistry Tissue Staining

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Title: Optimization Strategy For Fluorescence Multiplex Immunohistochemistry Tissue Staining

Background: Recent insights into cancer immuno-biology and the role it plays in response to therapy have fueled the need for tools that reveal multi-parameter cell level information in intact tissue sections, a need that is not adequately served by traditional one or two color immunohistochemistry (IHC). To address this need, manual and automated multiplexed immunofluorescence (fIHC) techniques are now being used in the immuno-oncology space. fIHC assays are designed to simultaneously measure multiple proteins at a cellular and sub-cellular level in FFPE tissue sections, retaining spatial arrangement and tissue architectural context, lost with other methods such as flow cytometry. We describe a novel optimization strategy to achieve quantitative, balanced, reproducible, and specific multiplex fIHC staining using both manual and automated approaches.

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