Automate Your Complete Cancer Research Workflow with Phenoptics™

Download our poster presentation from this year’s ABRF Annual Meeting to learn more about how you can completely automate your cancer research.

Title: A Fully-Automated Novel Method to Stain and Image Multiplexed Immunofluorescent Tissue Samples

Abstract: Cancer Immunotherapy research has increasingly leveraged knowledge about the tumor microenvironment for the development of novel therapeutics and targets. Advancing our current understanding of the tumor microenvironment will involve continued characterization of the interactions that occur among immune cells and cancer cells that reside within the tumor and its periphery. Fluroescent multiplex immunohistochemistry assays are uniquely suited to characterizing and quantifying these complex interactions in situ. Here we describe a robust, fully-automated 7-color fIHC method that would significantly shorten the Opal™ procedure from days to overnight. We coupled this automated staining procedure with a multispectral imaging system for simultaneous detection of up to 6 tissue biomarkers plus nuclear counterstain, providing the ability to visualize interactions between specific immune and cancer cells withing the context of the tumor microenvironment.

Phenoptics™ Quantitative Pathology Research Solution:
Vectra® Polaris™ Automated Quantitative Pathology Imaging System – multimodal digital pathology instrument integrating multispectral analysis and automated slide scanningintegrates both multispectral analysis and automated slide-scanning for faster, better quantification of up to 6 biomarkers
Opal™ Multiplex Automation IHC Detection Kits – transforms the traditional multi-day protocol into a high throughput solution that can be run overnight

Download Our Poster Presentation