Webinar: Developing and validating a multiplexed immunohistochemical/immunofluorescent assay for cancer immunology

Missed our live webinar? Catch up on what you missed by watching it on demand. This webinar will examine valid strategies for multiplexed analyses of immune-related protein and mRNA targets in human malignancies. Topics include: approaches for methods standardization, use of multiplexed fluorescence to interrogate the biological role and clinical significance of immune cell populations and immunotherapy targets in cancer.

Development of effective anti-cancer immunotherapies requires careful evaluation of the tumor immune microenvironment. Traditional techniques for tumor analysis, such as chromogenic immunohistochemistry and flow cytometry, have prominent limitations. Multiplexed quantitative fluorescence permits simultaneous detection of diverse targets within FFPE tissue sections, while preserving spatial context and quantitative output. 

Speakers:
Janis Taube, M.D, M. Sc

Director, Division of Dermathopathology Associate Professor of Dermatology at The Johns Hopkins Hospital
Clifford Hoyt

Oncology Fellow, Director of Applications and Services PerkinElmer, Inc
Summary:
During this webinar you will:
  • Gain a clearer understanding of multiplex IHC/IF and how it can be used to assess cell-level immuno-biology in intact FFPE tissue sections
  • Learn about an assay optimization approach developed collaboratively by Johns Hopkins and PerkinElmer
  • Engage in a practical discussion of how to develop and optimize multiplexed IHC staining protocols

View our webinar on-demand