Phenotypic Analysis of Hypertrophy in Human iPSC-Derived Cardiomyocytes

Learn how a phenotypic screening assay to study time-dependent effects of endothelin-1-induced hypertrophy was set up using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes.

Cardiac hypertrophy, characterized by abnormal enlargement of the heart muscle, is a major health risk where new approaches to drug discovery are needed.

Summary
Learn how you can:
  • Easily analyze three characteristics of cardiac hypertrophy: proBNP, actin re-organization and nuclear area, in a single, robust phenotypic assay.
  • Differentiate between early and late effects of endothelin-1 on hiPSC-derived cardiomyocytes.
  • Overcome the challenges of analyzing densely-growing cells by quantifying texture features, without the need for single cell segmentation.

Application Note