Webinar: HCS for Predictive Toxicology and in vitro Pathology Using Simple 3D Microtissues

This webinar will describe the integration of biology and engineering to devise simple, high-throughput 3D human microtissues as predictive biology platforms that reflect human physiology and disease, solving fundamental questions of adverse biological response with the goal of modernizing toxicity and drug candidate testing.

A key aspect of this approach is the implementation of “in vitro pathology”, making use of quantitative metrics from reconstructed 3D microtissue images to discern adverse biological effects. This strategy is currently being used to develop 3D microtissues of a variety of human tissues, exploring the utility of single cell lines, co-cultures of cells, and human induced pluripotent stem cells. The goal is to access a wide variety of cellular behavior, characterizing responses across the biological landscape using simple and rapid techniques.

Presenters
Kim Boekelheide, M.D., Ph.D.
Professor of Pathology and Laboratory Medicine
Brown University School of Medicine
Joe Trask
Senior Field Applications Scientist
PerkinElmer
Summary
In this webinar, learn about:
  • The tension between assay simplicity and biological complexity in designing 3D microtissues
  • Development of a human breast cancer MCF-7 3D microtissue model as an initial example of the approach
  • The definition of in vitro pathology and its application in quantifying the effects of estrogenic exposures in the MCF-7 3D microtissue model
  • Challenges and opportunities that arise with primary cells, co-cultures, and induced pluripotent stem cells

Webinar On-Demand