Case Study: Improving the Throughput of a Neuroprotection Assay Using the Opera Phenix High-Content Screening System

In this case study, we show how a previously described neuroprotection assay can be easily and directly transferred to the Opera Phenix™ high-content screening system, with a 4-fold decrease in acquisition time.

For the high-content neuroprotection assay, primary rat neurons are co-cultured on top of rat-derived astrocytes. To induce axon degeneration experimentally, NGF is withdrawn, leading to neuronal cell death, while astrocytes remain healthy. This can be captured by two readouts: the total axon area and the total number of nuclei. Neuroprotective drug candidates lead to an increase in the total axon area while keeping the number of nuclei (astrocytes) constant. Decreasing nuclei counts indicate a cytotoxic effect.

Summary
Learn how:
  • The Opera Phenix system has been applied in the field of neurodegenerative diseases
  • In this assay, the Opera Phenix system is 4 times faster than the previous Opera® system
  • Primary neuron morphology is analyzed in a straightforward approach using Harmony software
  • Careful assay optimization can increase throughput, and minimize the data burden, without compromising assay performance

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Case Study

Improving the Throughput of a Neuroprotection Assay Using the Opera Phenix High-Content Screening System