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FRET-based Quantification of Protein-Protein Interactions using the Opera Phenix High Content Screening System

In this webinar, you will learn how PPIs can be studied at higher throughput using a FRET-based assay on the Opera Phenix™ High Content Screening System. By taking advantage of simultaneous confocal imaging on the four cameras of the Opera Phenix system, we established a fast and robust FRET-based assay to measure the interaction of the anti-apoptotic protein Bcl-XL with the pro-apoptotic protein Bad.

During the webinar, the expert speakers:

  • Discuss how protein-protein interactions can be studied using FRET as an imaging technology
  • Show how a FRET-based assay can be done at higher throughput using the Opera Phenix High Content Screening System
  • Explain the benefits of the proprietary Synchrony™ optics technology for FRET-based assays
  • Describe how to perform ratiometric quantification of FRET efficiency on a pixel-by-pixel basis using Harmony® High Content Analysis Software

Simone Schicktanz
As experienced product manager for PerkinElmer’s high content screening portfolio, Simone Schicktanz has been closely involved with the development of the Opera Phenix High Content Screening System and the Harmony Image Acquisition and Analysis Software. Simone holds a master’s degree in Molecular Life Science from the University in Lübeck and previously worked as application scientist at PerkinElmer, developing a number of assays using a variety of PerkinElmer’s high content equipment. Prior to joining PerkinElmer, Simone Schicktanz worked at the Fraunhofer Institution for Marine Biotechnology where she contributed to the development of a novel cell tracking algorithm.

Jacob Tesdorpf, PhD
As portfolio director for high content instruments, Dr. Tesdorpf leads the global product management and application development for high content screening and live cell imaging at PerkinElmer. He holds a doctorate in biochemistry from Hannover University and did research on nuclear hormone receptors at the Max-Planck-Institute. Dr. Tesdorpf held several imaging related sales and marketing roles at Molecular Dynamics, Amersham Biosciences and GE Healthcare looking after proteomics, microarrays, pre-clinical imaging and high content analysis software. Prior to joining PerkinElmer in 2010, Dr. Tesdorpf headed the marketing group at 5 PRIME GmbH, a supplier of nucleic acid purification and amplification products.

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