Optical and microCT Imaging of Bacteria, Viruses, and Tumors

On Demand Webinar

The key to cancer therapy utilizing microbes is the ability to track the spread and replication of the bacteria or viruses within the body over time. A number of strategies employing IVIS® optical imaging for tracking reporter constructs in different models, from in vitro through to clinical samples have been developed. For bacterial work, novel lux reporter constructs for luminescence imaging of different genera in vitro and in vivo in mice have been generated. This has permitted 2D and 3D whole body imaging for a wide range of both invasive and non-invasive species in different anatomical settings– in tumors, various deep organs, and even within the gastrointestinal tract of live animals.

For eukaryotic work (tumors or viruses), luciferase tagged constructs permit similar imaging, the ability to monitor different luminescent reporters simultaneously, providing highly detailed monitoring of infections and host responses has been demonstrated.

Additionally, techniques for luminescence imaging of microbes and gene delivery in patient tissue ex vivo to provide clinically relevant data have been developed.

Examples of temporal and spatial analyses of various luminescent and fluorescent reporters in vivo, ex vivo and in vitro were described.

Presented By
Mark Tangney PhD.
Principal Investigator, Cork Cancer
Research Centre, University College Cork, Ireland

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Original Broadcast Date: 8 October 2013

Duration: 48 minutes