Technical Note: Key Success Tips to Performing B-Arr2 Recruitment Assays

フォームを入力いただくとダウンロードいただけます。

Key Success Tips to Perform B-Arr2 Recruitment Assays by Using the B-Arr2 Recruitment Kit With GPCRs

β-arrestin 1 and β-arrestin 2 are intracellular proteins playing central roles in GPCR signaling pathways by regulating agonist-mediated GPCR mechanisms such as internalization, where they act as endocytic adapters recruiting the GPCRs to the adapter protein complex 2 (AP-2) in clathrin-coated internalization pits. Different modes of arrestin-mediated internalization occur depending on receptors, cell types, and expression levels of β-arrestins and their partners.
The B-arr2 recruitment kit (PerkinElmer #62BDBAR2PEB/C) is a highly sensitive and specific tool for monitoring the cellular interaction between β-arrestin 2 and AP2 as a reading of β-arrestin 2 recruitment at the GPCR. It is designed to work in cells expressing both partners and overexpressing a GPCR. The assays are compatible with all classes of GPCRs and all types of GPCR/β-arrestin interactions where β-arrestin 2 interacts with AP2.

Read This Now