Guides: Optimizing Gs and Gi Agonist and Antagonist Assays

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Guide to Optimizing Antagonists of Gαs

The proper optimization of assay conditions is essential to ensure you obtain the best reagent use and performance. In the case of GPCR signaling, the most suitable assay conditions are dependent on the GPCR coupling (Gαs or Gαi) as well as the pharmacological characteristics of the compound being studied (agonist or antagonist). The optimal assay conditions will also hinge on the particular cell line used for the assay. For each setup, a number of optimization steps can be implemented that will ensure the best, most accurate results are obtained.
Drawing on hundreds of data sets and cases established by Cisbio’s scientists over the years, our different manuals provide the GPCR community with the most up to date guidelines for the optimization of Gαs and Gαi/o GPCR assays.

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