BRD4 is involved in gene transcription and is frequently dysregulated in many types of cancer. It is one of the most studied proteins in the Targeted Protein Degradation space where a number of PROTAC® molecules are being developed.
This note describes a method for in-depth characterization of PROTAC®s with a no-wash platform comprising biochemical and cell-based HTRF assays, using BRD4 as a case study.