0 Technical Note | How to access true Kd values in PPI assays | Cisbio | LP

How to access true Kd values in Protein-Protein Interaction assays

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Investigate the dynamics of Interactions

Kinetic values are of the highest importance for characterizing protein-protein interactions. Studying them brings priceless insight regarding macromolecule binding. Accessing kinetic values is therefore essential.

This application note dives deep into the topic to provide key guideline and experiments for determining true Kd values in protein-protein interaction assays with HTRF.

Benefit from an expert's insight on:

  • Handling HTRF data
  • Determining Kd
  • Investigating Prot-Prot Interactions